2,4-D Solution 
	2,4-D溶液 10mg/ml 
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						說明書 
					 
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						RT7299S 
					 
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						2,4-D溶液(10mg/ml) 
					 
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						5×1ml 
					 
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						1份 
					 
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	C8H8Cl2O3  MW: 221.04 
CAS:94-75-7 
	● Storage:-20℃
	● Specifications:
	Soluble…………10mg/ml in Ethanol
	Appearance…….colorless clear solution
	● Introduction:
	Typical working
concentration of 2,4-D is varied by application and plant species. It has been
reported that soybean treated with 2,4-D at concentrations between 5.0 and 10
mg/L stimulated callus growth, while at a much lower concentration such as 0.5
mg/L stimulated new cell formation and cellular enlargement for tobacco callus
tissue.2 Other concentration such as 1-3 mg/L of 2,4-D has been
reported to be effective in inducing callus formation of broad-leaf plants.1 Additionally, 2,4-D is often used together with IAA and L-tryptophan for callus
induction and suspension cultures of wheat, pearl millet and rice.2  2,4-D has also been used in seed testing for
the detection of Phoma lingam on Brassica spp when incubated on a blotting
paper that has been soaked with 0.2% 2,4-D solution.3 
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Reference:
	1. 
Witham, Francis H. 1968. Effect of 2,4-dichlorophenoxyacetic acid on the
cytokinin requirement of soybean cotyledon and tobacco stem pith callus
tissues. Plant Physiol. 43:1455-1457.
	2. 
George, E.F. 1993. Plant propagation by tissue culture. Part 1: The
Technology, 2nd Ed. Exegetics Ltd. Edington, Wilts. BA13 4QG,England.
3.  The
International Seed Testing Association (ISTA). International Rules for Seed
Testing ed. 2012. Annexe to Chapter 7 Seed Health Testing: Seed Health Testing
Methods.Bassersdorf,Switzerland.
d. Exegetics Ltd. Edington, Wilts. BA13 4QG,England.
	3. 
The International Seed Testing Association (ISTA). International Rules
for Seed Testing ed. 2012. Annexe to Chapter 7 Seed Health Testing: Seed Health
Testing Methods.Bassersdorf,Switzerland.